Inês Soares Marques

3 Questions 1 Answers 0 Followers

Questions related from Inês Soares Marques

Freezing HUVECs – recommended freezing medium, concentrations, and protocol?

Hello! I will be working with HUVECs and I want to prepare vials for freezing. I will be using the Endothelial Cell Medium - phenol red free from ScienCell (#1001-prf). What freezing medium and...

27 June 2025 5,918 3 View

Help with Thermocycler: Number of Cycles for cDNA Synthesis?

I am using the High Capacity cDNA Reverse Transcription Kit from Thermo Fisher and the GeneAmp® PCR System 9700 thermocycler to synthesise cDNA. The method specifies the following protocol: Step...

26 November 2024 4,573 4 View

How to Efficiently Prepare DNase I Mix for RNA Samples with Different Concentrations?

Hey! I am planning to extract RNA from my samples and subsequently remove any genomic DNA contamination using DNase I from Thermo Fisher (#EN0521...

15 October 2024 6,131 1 View