Indrajit Sahu

5 Questions 24 Answers 0 Followers

Questions related from Indrajit Sahu

How to chemically induce mitosis/APC activation in mammalian cells?

Hi All, Has anybody with experience in inducing mitosis by any chemical reagents..? My main aim is to activate APC/cdc20 complex along with the start of M phase. Currently only method I am...

12 December 2018 7,773 4 View

How to isolate native peptides from mammalian cell lysates for MS/MS application?

Hi All, Does anyone have experience with isolating native peptides from mammalian cells? Can anyone suggest a good protocol. The protocol I have is "Thermal denaturation of the cells at 80degree...

08 August 2018 2,740 1 View

What is the best method to prepare cell lysate from mice skin epidermal tissue?

I am trying to prepare cell lysate from mice skin epidermal tissue. I separated epidermis from dermis by 1X trypsin. Then reconstituted with RIPA buf, then homogenize, sonicate and centrifuge. But...

02 February 2017 6,665 5 View

What exactly is the biological meaning of immunoprecipitation? Your Views on this are needed..??

We all know "What is immunoprecipitation?" and we do it very frequently. But when we get the results come from WB or MS/MS we saw many interacting proteins (both direct and indirect). [No need to...

09 September 2016 9,487 1 View

What are the tricks for High Molecular weight protein transfer in western blot?

Hi All, Can anybody suggest what are the important points to consider when transferring high molecular weight proteins for western blot analysis. (Apart from low % of PAGE, less methanol and...

01 January 1970 8,043 6 View