Hi everyone, I am digesting my plasmid with SgrAI enzyme but it showed staractivity everytime?

My plasmid (pJET1.2) contains an insert of around 2969bp. The total size of plasmid 5942 bp. I am cutting it with AhdI, SgrAI and BssHII. Desired bands are of 2969, 1632 and 1432bp. I want to...

16 November 2020 4,890 4 View