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Questions related from Inci Basak Mustak
First, i PCR amplyfied my gene (~ 2500bp) and then cloned it to the bacteria (DH5@). Second, i digested it with restriction enzymes (blunt end, ~1300bp) and left flanking sites (~1200bp) inside...
09 September 2016 3,276 2 View
I have a sticky- end plasmid and want to create blunt- end from it. Which one is good for reaction, to use DNA Polymerase I or T4 DNA Ligase?
05 May 2016 9,491 9 View
I use commercial kits for extraction of plasmid DNA. But i don't have high plasmid DNA. For ex. i have tried MN (Macherey Nagel) NucleoSpin® Plasmid, Zyppy™ Plasmid (ZymoResearch) kits and got...
04 April 2016 3,999 11 View
Is there anyone know function about aroA gene which is used Salmonella vaccine?
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