Incheol Ryu

4 Questions 5 Answers 0 Followers

Questions related from Incheol Ryu

How can I explain different effect of overexpression on exogenous end endogenous mRNA?

Recently, I've got very strange phenomena. When I transfected a plasmid expressing protein 'A' with a plasmid expressing reporter mRNAs, which harbor specific 3' UTR, into HeLa cells,...

26 November 2015 8,474 2 View

Is there a step to stop during preparation of FACS samples for measurement of DNA?

I want to examine an effect of a chemical drug on cell cycle progression of HeLa cells. So, I'll prepare for the sample to measure the DNA contents via FACS analysis. During sample preparation, is...

09 June 2015 2,376 5 View

How can I detect phosphorylation of a gene of interest?

I am studying phosphorylation of a gene of my interest. In convenience, I call it “gene A.” At first, I have found that a threonine residue followed by proline in “gene A” is phosphorylated under...

17 February 2015 3,949 8 View

Is anyone familiar with formaldehyde crosslinking?

Recently, I have some troubles in formadehyde crosslinking followed by immunoprecipitation assay. Actually, my experimental purpose is to see whether a protein factor(s) could form a complex with...

27 November 2014 6,980 3 View