Iñaki Milton Laskibar

5 Questions 20 Answers 0 Followers

Questions related from Iñaki Milton Laskibar

Which ULK1 phopsphorylation should I use to analyze autophagy in energy restriction conditions?

Dear all I want to study the autophagy levels in liver samples of wistar rats under a 20% energy restriction. Among other autophagy "markers", I will tray to measure the ULK1 phosphorylation state...

12 December 2016 320 2 View

How can I measure the PGC1 alpha Thr178 phosphorylation in rat liver tissue?

Hi all I am working with rat liver tissue nuclear extraction. After I have measured the caetylation rate of the PGC1 alpha, now I would like to measure the Thr178 phosphorylation, in order to...

10 October 2016 4,621 2 View

Which is the acetylated lysine band that I have to use?

Hi all I have a problem with the PGC1 alpha/acetylated lysine western blot (with rat liver nuclear extracts). Once I get the reading of the PGC1 alpha bands (with no problem, abcam ab54481), and...

09 September 2016 8,437 5 View

Which could be the reason of an improvement in basal glucose and glucose tolerance test, without changes in HOMA-IR after resveratrol administration?

Hi all I am working in my PhD, and I have a problem. When comparing the data from my control group with the data of a group treated with resveratrol I find that: - No differences are observed...

03 March 2016 9,675 17 View

Can anyone give advice about problems with pAkt antibody (Ref # SC135650)?

Hi all I´m trying to quantify pAkt levels in rat gastrocnemius muscle. I´m using the antibody dilutions proposed by the manufacturer (Santa cruz, 1:1000), and I´m getting very "weak" and...

10 October 2015 3,461 4 View