Ina Buchholz

4 Questions 12 Answers 0 Followers

Questions related from Ina Buchholz

Can iodoacetamide reduce disulfide bonds?

Hi guys, This is a question for experts on protein cystine / thiol chemistry. Can iodoacetamide (under any condition) cleave disulfide bonds of a protein without addition of a reducing agent...

27 November 2018 2,353 3 View

TNBSA for quantification of free NH2 in proteins?

Hi guys, I'm currently working on the modification of Lys residues and need a quantitative assay to proof the content of free NH2 afterwards. Is there a good protocol with TNBSA...

12 June 2017 9,059 1 View

Methods for determination of protein tertiary structure

Dear all, which method would you prefer to determine changes in protein tertiary structure (no alteration in secondary structure)? I don't have the possibility for NMR, X-ray or SAXS. Thanks =)

27 April 2017 1,084 12 View

How to avoid fluctuations in tryptophan protein fluorescence?

Hi, this is a question to all experts on protein fluorescence: Currently, I'm trying to measure the trp fluorescence of a protein in buffer. It shows a nice signal, but unfortunately, results are...

21 February 2017 8,468 14 View