Why always there are two bands on colony PCR after cloning shRNA plasmids?

Hello. I recently cloned some plasmids for shRNA. I ordered PAGE-purified shRNA duplex oligo, and inserted it in lentivirus vector for shRNA(addgene cat.no. 8403, pLKO.1-puro) using AgeI and MluI...

03 September 2021 4,979 10 View