Imam Hossen

3 Questions 4 Answers 0 Followers

Questions related from Imam Hossen

I would like to perform HPLC/MS of a plant extract. Can anyone suggest me with the method of doing it?

I have hexane fraction of a plant leaf. I have sub-fractioned it by running on normal phase silica column. Now I want to run those sub-fractions in analytical HPLC/MS with PDA detector. Can any...

16 August 2018 8,356 5 View

What is the best way to count cells and determine cell viability of Caco-2 and raw 264.7 macrophage cells?

I am learning cell culture and while reading about counting cells and viability percentage, I am confused. Some recommend use of Hemocytometer and others tell about MTT. Hemocytometer is highly...

04 August 2018 5,228 1 View

Which cell type is better for assessment of in vitro intestinal permeability assessment, Caco-2 or Raw 264.7 macrophage?

I want to test the effect of certain phytochemical on cell types induced with certain chemicals like LPS, interferon gamma or TNF alpha. I would like to measure the TEER and other related...

13 May 2018 5,707 3 View