Imam Hossen

5 Questions 5 Answers 0 Followers

Questions related from Imam Hossen

I want to study advanced lipoxidation end products (ALEs) and their impact on gut health. Can anyone kindly suggest me the standard model?

I would like to perform both in vitro and in vivo experiment. But i can't understand the established models to perform my experiments. Care to share the models if anyone working on it or have...

28 March 2023 5,927 1 View

How to mark C57BL/6N mice?

I am about to do an experiment with C57BL/6N mice, but having difficulties to mark them. I tried several types of permanent marker to mark them like we used to mark Balb/c, but it's not...

19 June 2019 559 4 View

I would like to perform HPLC/MS of a plant extract. Can anyone suggest me with the method of doing it?

I have hexane fraction of a plant leaf. I have sub-fractioned it by running on normal phase silica column. Now I want to run those sub-fractions in analytical HPLC/MS with PDA detector. Can any...

16 August 2018 8,448 5 View

What is the best way to count cells and determine cell viability of Caco-2 and raw 264.7 macrophage cells?

I am learning cell culture and while reading about counting cells and viability percentage, I am confused. Some recommend use of Hemocytometer and others tell about MTT. Hemocytometer is highly...

04 August 2018 5,258 1 View

Which cell type is better for assessment of in vitro intestinal permeability assessment, Caco-2 or Raw 264.7 macrophage?

I want to test the effect of certain phytochemical on cell types induced with certain chemicals like LPS, interferon gamma or TNF alpha. I would like to measure the TEER and other related...

13 May 2018 5,741 3 View