Ilse Flores

3 Questions 1 Answers 0 Followers

Questions related from Ilse Flores

Why are there two bands in some of my PCR product and why are bands for restriction digest large?

I nucleofected iPSCs from one patient line, grew colonies and attempted to genotype them. i Had previously run a PCR gradient to determine the best Tm for the primers. most of the PCR products...

06 October 2022 5,774 0 View

Counting mutant huntingtin inclusions using ImageJ?

I've run IHC (using EM48 as primary; ABC kit; visualized with DAB; Nissl counterstain) on mouse brain sections (striatum). How do I count mutant huntingtin inclusions in these sections using ImageJ?

02 April 2018 8,474 1 View

What are the best TLR4 & MyD88 antibodies one could use in co-immuniprecipitation (lysed brain tissue)?

We will be lysing mouse brain tissue for the co-IP of TLR4 and MyD88, followed by a western blot.

24 October 2017 7,756 2 View