Ildikó Tándor

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Questions related from Ildikó Tándor

Could anybody help me? What is the problem with my protocol of lymphocyte separation? Or our patients have got too high red blood cell number?

In general the separation works without problem, but sometimes the reaction is not convenient.I think the red blood cell get stuck in the gradient. If I tried to discharge the cell layer, I...

14 July 2016 2,450 14 View

Why can my primer amplify gDNA, if I designed it in exon-exon junction?

I designed one primer pair in exon-exon junction. My forward primer is in exon, but the reverse primer is in exon-exon junction. My primer designer is NCBI-PrimerBLAST. My PCR product was run in...

28 July 2015 6,543 8 View

I am working urine after prostatic massage, and I would like to study transcript from urine. How can I lyse cells without RNA degradation?

I am working with ~50 ml urine after prostatic massage, and I use a RNA stabilizator, but I would like to study transcripts from prostate cells in urine.Do I need to lyse the cells?

16 May 2014 5,628 2 View