Can multidrug resistance-associated proteins cause uneven calcium dye loading in acute hippocampal slices?

I am new to the field of calcium imaging. I used OGB-1 AM dye at 2 uM concentration for 1 hour at 35 C in adult rat hippocampal slices. Only neurons in the stratum radiatum were loaded and the...

18 February 2015 1,135 2 View