Ilario De Toma

2 Questions 1 Answers 0 Followers

Questions related from Ilario De Toma

What is the best way to disrupt and homogenize brain areaa for RNA extraction?

I have brain frozen mouse brain areas (frontal cortex, hippocampus and hypothalamus) and I would like to extract RNA from them using the Trizol or the RNeasy kit. Is it sufficient to use a insulin...

27 June 2016 275 2 View

Is it possible to freeze FACS sorted cells for future -omic studies?

Hi everyone, I am about to collect FACS-sorted cells to perform in parallel a transcriptomic and an epigenomic study. To this purpose I need to split my sorted cells into three tubes and perform...

27 June 2016 4,277 2 View