Ilaria Scurci

4 Questions 2 Answers 0 Followers

Questions related from Ilaria Scurci

Does anybody know a sulfate-free media?

I perform experiments on removal of sulfation by sodium chlorate from the target receptor. For that reason, I need to incubate cells in sulfate-free media. Does anybody know any media with this...

02 February 2017 4,862 2 View

Why by CRIPR-Cas9 I get only point mutations and how can I induce disruption of my protein of interest?

I performed CRISPR/Cas9 in CHO cells to KO the protein Arrestin3. I transfected cells with a single plasmid containing gRNA - Cas9 - YFP. Then I sorted for YFP and I screened clones by sequencing....

01 January 2017 6,060 2 View

How to disrupt glycosylation on receptor expressed on cell surface?

I would like to remove glycosylation from the C-terminal part of the GPCR receptor CCR5 to do further study on ligand binding. Does anybody know if it exist an enzyme that can be added to the cell...

01 January 2017 5,273 4 View

How to design primers for PCR screening of CRIPR KO in CHO-K1 cells?

I have to screen CHO-K1 clones to search for KO of my gene of interest by CRISPR. For this purpose, I need to amplify the exon with the expected mutation by using primers specific of that region....

11 November 2016 4,709 0 View