How to perform stable transfection of pcDNA3.1(+) using Lipofectamine 3000?

I wish to make stable Huh7 or HepG2 cells with my gene of interest using pcDNA3.1(+). I´m using Lipofectamine 3000 kit and Geneticin selection 72h post transfection but the cells tend to loose the...

14 January 2022 3,883 3 View