Ijsbrand Reyneveld

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Questions related from Ijsbrand Reyneveld

What is the shelf-life of freshly prepared PBS + 0,5% BSA?

For each experiment that requires PBS + BSA (PBSA), I prepare it freshly and store it at 4oC if there are any leftovers. But for how long can it actually be stored? Does the shelf-life differ with...

12 June 2018 7,699 6 View

What culturemedium, or how to compose one, is the most biocompatible for culturing human PBMCs?

Currently, I'm culturing my cells in RPMI1640 or AIM-V (Life Tech.) + 10% FCS or 5% PHS respectively for ten days tops.

18 December 2015 6,293 3 View

What is the optimal flowrate for the analysis of CFSE labeled T-cells?

Currently, I'm optimizing a Celltrace-CFSE assay. I want to have very distinct peaks in my flowcytometric histograms to analyze proliferation percentages. However, to receive such peaks, I need to...

26 November 2015 1,177 7 View