Igor Mazheika

3 Questions 14 Answers 0 Followers

Questions related from Igor Mazheika

How to bias competitiveness in favor of insertion during ligation?

I insert a sequence into the plasmid at the PspLI and BamHI restriction sites. The sticky ends of these restriction enzymes share two of four identical nucleotides. This likely explains why I get...

28 June 2025 632 13 View

Why don't I have a PCR product with proofread polymerase?

Dear Colleagues, I have the following problem: I’m trying to amplify a cassette with a resistance gene (about 2 kb) from the yeast genome, so that I can then insert it into a plasmid. With Taq...

04 July 2024 170 11 View

What is color of 1% stock solution of DAB tetrahydrochloride?

We dissolve 3,3′-diaminobenzidine tetrahydrochloride in water for immunohistochemistry and the solution becomes dark violet. Is it normal? When dissolving diaminobenzidine (not tetrahydrochloride)...

18 January 2016 9,686 4 View