Ifor R Williams

2 Questions 11 Answers 0 Followers

Questions related from Ifor R Williams

What is the best method for recovering cells from 3D Matrigel cultures (enteroid cultures) so they can be used for RNA isolation and qPCR analysis?

Residual Matrigel can interfere with RNA isolation and downstream analysis. Some of the methods I've seen used for this application include BD's non-enzymatic Cell Recovery Solution, dispase and...

03 June 2014 8,951 21 View

Villin-cre transgene integration site: Can anyone lend their expertise?

Does anyone know which mouse chromosome the widely used villin-cre transgene (B6.SJL-Tg(Vil-cre)997Gum/J) is integrated into?

18 April 2014 8,859 7 View