How to avoid gelation/slime formation in muscle homogenate?

I am isolating mitochondria from rat quadriceps, using the following procedure: First I cut ~200 mg quadricep placed in 1 mL of ice-cold isolation buffer (Sucrose 100 mM, KCl 100 mM, Tris–HCl...

22 March 2024 1,984 0 View