Ibrahim Oguz

3 Questions 3 Answers 0 Followers

Questions related from Ibrahim Oguz

Some questions about Crispr/Cas 9 (T7 Assay, extra band expectation, positive control, alternative method to single cell isolation and expansion ?

We have psp cas 9 2a puro and esp cas 9 1.1 vectors. We are performing T7 assays for mutation detection. Do we have the possibility to use positive control? Do we have the chance to estimate how...

14 May 2018 5,446 4 View

Need help about some Crispr/Cas9 protocol pSpCas9(BB)-2A-Puro (PX459) V2.0 to HEK 293T Cells l ?

We are studying the Crispr/Cas 9. We have clonned our gRna into pSpCas9(BB)-2A-Puro (PX459) V2.0. Now, we are planing to do cell line experiments. Our cell line is HEK293T. Our crispr vector...

22 September 2017 676 5 View

Why does agarose gel look smear image and background composition ?

I isolated the my plasmids several times with isolation kits and to check , cut with restriction enzymes than run the agarose gel electrophoresis but ı got smear bands also background emission....

05 June 2017 3,117 2 View