Why does my IVT gRNA product gives HMW smear in gel?

We are using MEGAshortscript™ T7 Transcription Kit for transcribing gRNAs from linearized plasmid templates. (MSP3485 digested with HindIII) (https://www.addgene.org/140082/) We let the reaction...

02 July 2024 6,186 0 View