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Questions related from Iason Karyofyllis
So another PCR problem. I have to amplify a 3 kbp construct with high GC content. Generally all the constrcut isaround 63%. My construct consists of the pcagpromoter with the cmv enchancer and a...
30 November 2018 6,234 8 View
Dear all, I want to subclone a 779bp part from one vector to the other. I am cutting with EcorV-HF and MFeI. The bands of the digestions in the gel are clear.So I then Gel extract and try to...
03 May 2018 6,752 4 View
