Ian Smith

6 Questions 7 Answers 0 Followers

Questions related from Ian Smith

What company is the best for serial block-face scanning electron microscopy?

I am in the midst of doing a large scale study using serial transmission electron microscopy. It would be helpful if we had a scanning EM here at Texas A&M but we do not have one currently. We...

04 April 2018 4,246 2 View

What is the best method for coating slot-grids for TEM?

I currently coat my grids (copper-beryllium slotgrids) in a 0.28%PEI film. The advantage of PEI over traditional formvar is supposed to be for its strength, making it ideal for re-examination of...

01 January 2018 1,214 4 View

What software do you use to segment electron micrographs for reconstruction? Is it auto-mated?

Currently I use “Reconstruct Synapse Web”, made available by the Harris lab at the University of Texas at Austin. Although I am very familiar with this software and do enjoy using it, it requires...

12 December 2017 4,708 4 View

How well does the Perfect-Loop tool work for transferring thin sections (65nm) to coated, slot-grids for transmission electron microscopy?

I am trying to make the process of loading floating sections onto grids less difficult and more repeatable.

05 May 2017 8,767 3 View

What method do you use for picking up thin sections from water-boat with coated, slot-grids for transmission electron microscopy?

Currently I use the method of submerging the grid below the water surface and coaxing sections onto grid with hair-tools. The problem is that they often drift away and do not settle onto the...

08 August 2016 9,259 9 View

What is the best neuromuscular conference to attend for graduate students?

We all have symposia and conferences we attend, some better than others. As a graduate student it can be a challenge to decide which meeting is best for my own interests, especially considering...

01 January 1970 2,361 0 View