Ian Lidbury

2 Questions 12 Answers 0 Followers

Questions related from Ian Lidbury

How do I avoid lag phase for P. putida and P. fluorescens cultures in minimal medium when subculturing?

Although theese bacteria should be incredibly easy to cultivate, I am experiencing some trouble when subbing/ inoculating cultures for growth experiments. An over night culture of minimal medium...

31 March 2015 8,277 7 View

What is a good binding buffer for use with helix-turn-helix DNA binding proteins (GntR-type repressors)?

I'm looking for a good binding buffer to look at the binding potential of a repressor with a region of DNA. I am going to do a gel-shift assay to prove the binding. Any suggestions on an...

17 June 2014 6,160 1 View