Ian F Harrison

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Questions related from Ian F Harrison

Does anyone have a good rapid immunohistochem protocol for staining of GFAP positive astrocytes in mouse brain prior to laser capture microdissection?

We're looking to use laser capture microdissection to isolate astrocytes from mouse brain tissue. Has anyone worked with a rapid immunohistochemical stain for GFAP in mouse tissue which can be...

20 January 2015 6,712 1 View

Is anyone familiar with the use of TH as a housekeeper gene for delta delta Ct analysis?

I've run some qRT-PCR experiments looking at expression of TH (tyrosine hydroxylase) and a number of other genes of interest in mRNA sections from human brain tissue. I have already run delta...

31 August 2014 2,470 2 View