Hyojeong Chu

2 Questions 3 Answers 0 Followers

Questions related from Hyojeong Chu

GFP is expressed in rt-pcr, but why can't it be seen in plants?

Hello :) I'm studing an unknown gene(about 4kb) of Arabidopsis thaliana. To observe the phenotype when the gene was overexpressed in plants, the pBI vector (35S-unknown gene-GFP) was transformed...

22 April 2024 3,832 4 View

How do I design a molar ratio for gibson assembly?

Hello, I am conducting a gibson assembly with 2 inserts and 1 vector. vector size = 13kb insert A size = 1.7kb insert B size = 4.2kb So, I tried gibson with 1:3:3 (vector:A:B) molar ratio. Is this...

27 March 2024 4,404 1 View