Hyo-Min Kim

3 Questions 8 Answers 0 Followers

Questions related from Hyo-Min Kim

Any advice in pelleting from FACS sorted cells ?

Greetings, I am currently sorting a rare population of cells by flowcytometry. Getting very small amount of the sorted cells in a volume of 5 ml (including sheath buffer and 2%FBS/PBS). Tried so...

08 December 2015 5,316 12 View

What is the appropriate way to use diluted Matrigel for epithelial cell culture?

I saw some protocol that used diluted Matrigel (1 to 1 Matrigel with DMEM/F12.) for epithelial cell cultrue. I know that diluted matrigels are used for plate coating, however is there someone who...

22 June 2015 4,625 3 View

Any suggestion to safely remove Matrigel during small intestinal crypt culture?

Planning to use Ice cold PBS protocol (Mahé, Maxime M et al. “Establishment of Gastrointestinal Epithelial Organoids.” Current protocols in mouse biology 3 (2013): 217–240. PMC. Web. 19 June...

19 June 2015 2,361 5 View