Hye Eun Chun

4 Questions 5 Answers 0 Followers

Questions related from Hye Eun Chun

Ways to reduce background noise for Western Blot ?

Hi everyone, Currently, I am running a western blot where I cut my membrane into 5 different pieces and individually incubate them with their respective primary and secondary antibodies. I have...

02 February 2020 6,885 9 View

Cutting Western blots with proteins with similar molecular weights?

Hello everyone, Currently, I'm trying to detect five different proteins on a single western blot gel. To do this, I load 5 of the lanes with my sample lysate and I cut it vertically along each of...

01 January 2020 3,607 2 View

Problems with using PVDF membrane in a Western Blot?

To conduct my western blot, I used a PVDF membrane which I activated by wetting the membrane in methanol for 1 minutes ( change from opaque to translucent), placing the membrane in Milli Q water...

01 January 2020 10,106 5 View

What could cause these "frowning" bands seen in electrophoresis (SDS-PAGE)?

As part of my western blotting procedure, I ran my SDS-PAGE electrophoresis. Usually, I load in 2 lanes, 1 ladder (spectra broad range protein ladder) and my sample (sample lysate + Laemmli...

10 October 2019 2,127 16 View