Hye Eun Chun

3 Questions 4 Answers 0 Followers

Questions related from Hye Eun Chun

Problems with using PVDF membrane in a Western Blot?

To conduct my western blot, I used a PVDF membrane which I activated by wetting the membrane in methanol for 1 minutes ( change from opaque to translucent), placing the membrane in Milli Q water...

22 January 2020 5,219 6 View

Cutting Western blots with proteins with similar molecular weights?

Hello everyone, Currently, I'm trying to detect five different proteins on a single western blot gel. To do this, I load 5 of the lanes with my sample lysate and I cut it vertically along each of...

12 January 2020 8,822 3 View

What could cause these "frowning" bands seen in electrophoresis (SDS-PAGE)?

As part of my western blotting procedure, I ran my SDS-PAGE electrophoresis. Usually, I load in 2 lanes, 1 ladder (spectra broad range protein ladder) and my sample (sample lysate + Laemmli...

31 October 2019 6,797 16 View