Hyder Mir

4 Questions 6 Answers 0 Followers

Questions related from Hyder Mir

Does anyone have a protocol available for the isolation/selection of CRISPER/ KO CD34 cells from wild-types ?

I performed an experiment involving the knockout (KO) of XYZ gene in CD34+ cells, followed by their differentiation into MKs. I am now seeking a reliable protocol for sorting the KO cells from the...

31 January 2024 8,358 0 View

False positive results in influenza testing. Need help ?

I have been encountering false positive results while processing the samples for influenza typing and subsequent subtyping. I was wondering if real time rt pcr (without calibration) could be the...

14 January 2018 628 6 View

Influenza A probe giving peak in every well ?

there was some problem with my influenza A probe as it was giving a peak (>35 ct) in each well, even in confirmed negative samples... i thought that probe must have degraded. So i ordered a...

08 October 2016 3,712 2 View

What is the amount of DNA required for telomere measurement?

I wanted to measure the telomere length in COPD patients using whole blood. Im using Qiagen DNA extration mini kit which yields around 3–12 µg of DNA. i was wondering is the amount of DNA enough...

16 June 2015 2,069 5 View