Hw Yn

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Questions related from Hw Yn

Where the protein after T2A self-cleavage peptide will be localized ?

Hi, I am attempting to co-express GFP using a bicistronic vector that contains the T2A peptide. (CD8 alpha signal peptide - GOI - T2A - GFP) I am concerned that, since I am using the CD8a signal...

13 January 2024 8,908 2 View

How do I increase the persistency of CAR expression after lentiviral transduction?

Hi, I am currently working on CAR cell therapeutics development and I am using lentivirus as a vector for transgene delivery. As per my understanding, lentiviral transduction is known to give...

14 August 2023 6,938 1 View

Does ecto-domain of CAR have an effect on CAR display?

Hi, I am doing CAR-NK research and I have a huge problem with that. I switching the ecto domain of my CAR (anti FITC ScFv to anti HER2 ScFv). I analyzed the sequence of CAR and prepare the DNA...

27 July 2020 5,983 1 View

How I produce lentivirus with larger titer?

Hi, I have some questions about my lentivirus. 1. When I measure the titer of virus, I use 2 fold diluted virus from 1:100 to 1:1600 and transduction the virus into HEK293FT cells (On 24 well...

16 March 2020 4,700 2 View