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I have some samples I left in sucrose for a couple of weeks after perfusion and was wondering if these samples are still salvageable? I perfused the brain, then they were fixed in 4% pfa for 24...
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Hi. I am doing immunohistochemistry staining on mice tissue. We perfuse the mice and place the brain in 4% PFA O/N then switch to 48hr in 30% sucrose (or until the brain sinks). However I am...
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