Please help! Double bands when running WB for alpha-SMA and beta-actin on the liver sample, BUT NONE OF THEM WAS SPECIFIC?

Hello, I'm running western blot for alpha-SMA and beta-actin from the liver sample. Incubating in reducing buffer, 80oC for 15 mins 1st antibody: alpha-SMA ab15734 (1:1000), rabbit polyclonal...

06 February 2018 8,348 4 View