Huimin Yap

3 Questions 4 Answers 0 Followers

Questions related from Huimin Yap

Has anyone done co-IP for mTORC2 complex before?

I'm planning to study the effect of my compound on the mTORC2 complex. I noticed most of the paper reported that they immunoprecipitated mTOR and immunoblotted with Rictor. If I do it the other...

06 June 2016 2,244 2 View

Has anyone done a gel contraction assay with human bronchial smooth muscle from lonza?

I want to determine the drug effect in inhibiting human bronchial smooth muscle contraction induced with histamine. Currently, I tried it with 1. 0.5 X 10^6 cell per well in 24 well plate with...

01 January 2015 8,842 4 View

Have anyone encounter problem with human bronchial smooth muscle cell seeded in 96 well plates?

Hi, I'm culturing human bronchial smooth muscle cell (Lonza). Recently, I'm having problem with this cell when I seeded them in 96 well plates (BD brand). After serum starved for 1 day, I change...

11 November 2014 7,964 2 View