Hui Yee Ng

2 Questions 2 Answers 0 Followers

Questions related from Hui Yee Ng

Why do I always get multiple MyoD band after Co-IP?

I am currently working on Co-IP of NRIP and MyoD by overexpressing both proteins in HEK293T cell. The question is I cannot get a specific band of MyoD. For my input, I think the protein level is...

07 April 2023 2,512 4 View

How to remove non-specific binding in co-immunoprecipitation?

I am working on co-immunoprecipitation of endogenous NRIP and NFATC1 protein in C2C12 myoblast cells. However, protein bands with the same molecular weight as our target were found in the control...

30 March 2023 2,132 2 View