Hugo Farne

4 Questions 2 Answers 0 Followers

Questions related from Hugo Farne

Sample size/power calculation for predictive biomarker study?

I have two groups of patients, responders (~75%) and non-responders (~25%) to a treatment. I want to look for a difference between them in a single biomarker (which I have not yet measured). The...

03 July 2019 3,513 3 View

Are CD3+CD4+ cells double positive for T-bet & GATA3 Th1 or Th2 cells?

I have been staining human samples from subjects with asthma, interested in type 2 inflammation. A proportion of the CD3+CD4+ T cells stain positively for both the Th1 and Th2 transcription...

24 June 2018 4,262 4 View

Flow cytometry for Innate Lymphoid Cells in humans: are CRTH2 & CD117 enough, or do I also need NKp44?

Hi I'm designing a flow cytometry panel including markers for Innate Lymphoid Cells, to use on human samples. As far as I can tell, they are: ILC1: CRTH2− CD117− NKp44− ILC2: CRTH2+...

16 November 2015 8,098 9 View

Can anyone help measure glucose in very low concentrations (0-100μM) in respiratory samples?

E.g. using HPLC or GC-MS. The samples come from a well-characterized population, work on which has been published before. We're based in London and happy to transport samples, help with the labor,...

28 May 2015 5,947 3 View