Huda Khalfan Al Brashdi

6 Questions 8 Answers 0 Followers

Questions related from Huda Khalfan Al Brashdi

How can I get two PCR products for the same sample using one marker (forward and reverse)?

I ran a PCR for seaweed samples using cox2-3 spacer Cox2 forward and cox3-Reverse, I got a PCR product around 360 bp (with sample 1,37) with both annealing temperatures 48 and 50 ͦC as it is shown...

11 September 2023 4,644 0 View

I got an error message :"Could not load profile- Invalid id format" when I used the MG-RAST for metagenomic study, How to solve this issue?

I registered for an online course (Bioinformatic Methods 1) from Coursera, in one of the labs we are dealing with the MG-RAST online server. Im searching for the "sequence Break down / predicted...

11 July 2023 8,829 1 View

How can I solve this problem, I got the "could not load profile - invalid id format " message when using MG-RAST server?

I'm having a question regarding the metagenomic analysis online server, I'm using it in one of the courses, and we are comparing two sequences, However, I start the analysis I got the following...

04 July 2023 537 0 View

How many many well-supported nodes are there (bootstrap scores>=70%) with the protein sequences analyzed in the phylogenetic tree (attached)?

Note that while bootstrapping has an element of randomness to it, there is fairly large difference between those nodes that are well-supported and the others. What it means that the bootstrap has...

22 June 2023 6,584 4 View

What is the bootstrap score for the P.marinus clade containing the 5 P.marinus strainsin the ML phylogenetic tree (attached) ?

What is the bootstrap score for the P.marinus clade containing the 5 P.marinus strains in the ML phylogenetic tree (attached) with aligned DNA sequences? Tree was constructed using Maximum...

20 June 2023 7,563 4 View

What are the reasons for PCR failure?

Hi every body, we have seaweed samples extracted using CTAB protocol, DNA quantity and Quality was good. We did PCR for the samples using ITs ribosomal DNA and rbcl marker. Positive control was...

19 April 2023 788 10 View