Truncated protein expression from codon-optimized sequence in E. coli — full-length not detected?

Hi all, I'm currently expressing a codon-optimized sequence cloned into pET-24a(+), with a C-terminal His-tag, in E. coli cells. The full-length protein is expected to be ~227 kDa. I induced...

25 March 2025 5,275 10 View