Huanhuan Xiao

3 Questions 5 Answers 0 Followers

Questions related from Huanhuan Xiao

What can I do if I knocked out one gene in zebrafish with no phenotype?

I knocked out a gene which is expressed in skeletal muscle, one is hsp40 and another one is a co-chaperone of hsp90a1. Previous study showed that hsp90a1 is specially expressed in skeletal muscle,...

13 December 2019 5,314 4 View

Why F0 germ cell has different mutations after sgRNA injection by CRISPR?

I knocked out a specific gene in zebrafish for study by the CRISPR cas9 system. In the F0 founder fish, there are about 80% of fish with mutations in a certain gene. For one fish with mutations...

01 January 1970 5,307 4 View

Can a mutant line without the nonsense RNA decay by CRISPR be viewed as a successful knockout?

Now I got two mutant lines of a gene in zebrafish. One line has nonsense RNA decay, which is knocked out in the middle exon(5th exons) of the whole gene(9 exons in total). And another line does...

01 January 1970 567 0 View