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Questions related from Huan Qi
All my proteins were expressed in the E.coli, and each one only 0.5 g. How to destroy the cells for following protien isolations? The CelLytic B 10X from Sigma was tested. However, about 50%...
21 June 2023 9,431 1 View
In my experiment, the size of dot blot on the NC membrane was different. Sometimes, the coffee ring was formed, in which the signal was less in the centre part. How to resolve this question?
05 August 2017 8,621 0 View
peptides were not immobilized on the NC membrane. Do the NC membrane need to activate before being used? By the way, any suggestions were welcome.
12 April 2017 8,480 2 View
Plenty of the coffee ring was formed in the printing microarray on the 3-D surface. How can I improve this result by avoiding the coffee ring?
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