6 Questions 13 Answers 0 Followers
Questions related from Hua Gai
Dear all, Interest in RNA-protein interactions is booming. In the protocols of CLIP and RIP, UV radiation is used to cross-link RNA binding proteins to the RNA that they are bound to. However,...
05 May 2018 5,019 4 View
Hi, everyone As we all know, a protein can shuttle between the nucleus and the cytoplasm. Whereas many proteins are selectively transported from the cytoplasm into the nucleus, most RNAs are...
04 April 2018 4,765 4 View
Hi,everyone I'm doing experiments on exosome. And Western blot is a well established method to detect exosome. The tetraspanins CD9, CD63 and CD81 are commonly used for exosome detection. I have...
06 June 2017 7,707 2 View
Hi, everyone When I try to make a histogram with error bar, I need the average and standard deviation(SD) calculated for one set of data first, and the error bar is represented by SD. However,...
09 September 2016 4,971 8 View
I run a touchdown PCR, and I found many bands in my no DNA template group and even other groups.In no DNA template group，there are 2 pairs of primers，and each primer is 20bp or 22bp. I run this...
08 August 2016 757 3 View
I am a learner about flow cytometry. I use Ficoll to isolate the mononuclear cells in mouse' spleen. And when I try to analyze the flow cytometry result, I find I have a question to ask. How to...
08 August 2016 2,987 10 View
