What's the main difference between #Tris-glycine #SDS-PAGE and #Bis-Tris #SDS-PAGE. How pH and ion strenght affect SDS-PAGE?

sometimes, there is some smear on the SDS-PAGE, this can be eliminated with a different running buffer or a different PAGE, or a different way of sample loading buffer. how these affect the SDS-PAGE?

10 August 2020 6,317 3 View