No classical response of CC2-DMPE/DiSBAC2(3) FRET after depolarization on HEK293 cells

Hi guys, I'm trying to use CC2-DMPE/DiSBAC2(3) FRET pairs to image membrane potential and firstly i just try to show classical increase in FRET ratio(460nm increase, decrease in 580nm) in HEK293...

07 July 2017 7,011 0 View