Howard Chan

2 Questions 4 Answers 0 Followers

Questions related from Howard Chan

What is the concentration of prey protein when using purified proteins in Gst-pulldowns?

I want to do a pulldown using 2 purified proteins. One is Gst-tag, the second is His-tag. I want to use the immobilise the Gst-tag protein on glutathione sepharose 4B beads, and try pulldown the...

10 October 2014 8,411 3 View

Are there protocols to pulldown a purified protein with a second purified protein?

Hi, I am working on protein-protein interactions. I currently have 2 purified proteins (a His-tag and a GST-tag) which is hypothesised to interact with each other. I want to test if these 2...

10 October 2014 4,766 6 View