Houyem Ouragini

2 Questions 2 Answers 0 Followers

Questions related from Houyem Ouragini

Can RNA from red blood cells be studied/extracted?

I want to study by RNA seq the transcriptome (mRNA and microRNA) of red blood cells (erythrocytes or reticulocytes). Can I do it (as there is no nucleus) and how can I extract it?

03 April 2024 3,543 1 View

Luciferase assay for gene-miRNA interaction: which vectors should be used?

To analyze gene-miRNA direct interaction, I want to perform a luciferase reporter assay. I plan to clone the 3’UTR of interest in pGL3 basic vector ( which is a promoter-less vector). Can I use...

24 November 2020 2,938 2 View