Horea Szedlacsek

5 Questions 6 Answers 0 Followers

Questions related from Horea Szedlacsek

SDS PAGE Blur in the lower part?

I'm using a standard SDS PAGE protocol, but since a month or so, some mysterious issues started to cause us trouble as seen in the first two pics (gel1 and gel2). The third picture is from a...

03 December 2020 5,001 5 View

SDS PAGE - nothing visible in the upper part, blur in the lower part?

I'm using a standard SDS PAGE protocol, but since a month or so, some mysterious issues started to cause us trouble as seen in the first two pics (gel1 and gel2). The third picture is from a...

03 December 2020 5,195 6 View

Scopolamine - Anxiolytic or anxiogenic?

Hello, I am encountering difficulties in understanding a specific issue in the role of muscharinic acetylcholine receptors (mAChR) in anxiety, as illustrated in the attached image. How can both...

02 July 2020 1,672 4 View

How do nervous impulses propagate due to depolarization by the AMPA receptors?

Hello. It is already common knowledge that AMPA receptors, upon binding glutamate, become open to Na+ ions wich flow into the neuron, thus causing the membrane to depolarize. Nevertheless, I...

04 March 2019 2,668 4 View

Enzymatic reactions which do not conform the Michaelis-Menten Model. What are the alternatives?

Hi, I'm trying to determine the kcat and Vmax for a dephosphorylation between a phosphopeptide (500 μM) and a phosphatase (50 nM) using a spectrophotometer and measuring absorbance at 282 nm....

08 May 2018 963 21 View