How to detach activated THP-1 cells (M0, M1, M2) from culture dish to not loose specific cell surface markers such as CD163, CD209, CD80 etc?

Following differentiation experiment (24h PMA 100 ng/mL -> 48h RPMI 10% FBS -> 72h IL-4 40ng/mL (M2) or LPS 50 ng/mL + INF 50 ng/mL (M1)) we found very difficult to detach cells for FACS...

29 March 2022 1,149 1 View