Hong Thai Le Nguyen

4 Questions 7 Answers 0 Followers

Questions related from Hong Thai Le Nguyen

Slot data in Differential Expression analysis in scRNAseq?

Hi everyone, Let me introduce my data: I am analyzing the single-cell RNA seq dataset. I'm gonna find the differential expression (DEGs) from different conditions in each cell type. In addition,...

26 September 2023 3,678 1 View

Protein size deviation from standard ladder when running sds-PAGE?

I have collected synthetic proteins from E.Coli. Then run SDS-PAGE. The problem is when calculating insilico its size should be around 64kDa. However, when running on the ladder, it is below the...

24 October 2022 3,280 3 View

Determination of Imidazole salt concentration in purified protein?

I performed protein purification through his-tag and Ni-Agarose. The result of this process is quite good but the problem is that I need to use this protein for the test with the substrate. Too...

18 October 2022 9,742 3 View

Mass of E. coli CD41 DE3 lysate preparation with sonication?

I am preparing a sonicate to destroy E.Coli CD41 DE3 cells. However, the sonicate efficiency does not seem to be high. I follow the following procedure: Dry biomass is mixed with 1.5 ml of lysis...

14 January 2022 9,845 2 View