Hong-su Park

3 Questions 8 Answers 0 Followers

Questions related from Hong-su Park

Why expression is significantly reduced or lost by some expression plasmids after a while?

Here is a question that I encounter when I express some genes cloned into epitope-tagged mammalian expression vectors. I know, for certain genes (or parts of genes) into certain vectors, they do...

09 September 2017 5,623 10 View

Can anyone help me with ASC oligomerization assay?

I have tried to detect ASC oligomers after overexpression but still struggling. I can see ASC monomers and just smears somewhere above 100 kDa, but not dimers, trimers and oligomers in WB.Any...

12 December 2015 5,696 6 View

How can I clone Caspase-1 from porcine alveolar macrophages?

Has anyone cloned the pro-caspase-1 gene from primary porcine alveolar macrophages? Or has anyone seen the mRNA expression by real-time PCR? For cloning, I treated the cells with LPS for 2 or 4...

06 June 2014 5,581 3 View