Hong Wang

4 Questions 2 Answers 0 Followers

Questions related from Hong Wang

Has anyone observed cell membrane fusion to huge cells with multiple nuclei after lentivirus transduction? how to avoid this?

I performed lentivirus transduction with my primary tumor cell (will form spheres) for 24 hrs and observed that the membrane of the sphere cells fused to huge cells. Anyone has same experience?...

08 November 2017 3,294 0 View

In general, how long does it take for lentiviral transduction to occur in primary cells?

I want to determine the shortest time that is enough for lentiviral transduction to occur in my cells

01 November 2017 7,245 4 View

Polybrene is toxic to my primary cell for lentiviral transduction. Any alternative to improve the transduction efficiency?

Without polybrene, the transductioin efficiency is very low in my primary tumor sphere primary cells

01 November 2017 9,793 6 View

For PCR with 72 bp primers (30 bp are flanking at the 5' end) to PCR a amplicon of 240 bp, how to decide the annealing temperature?

PCR condition

07 October 2017 7,239 3 View